外细胞/糖化白蛋白(糖蛋白)/甘聚糖:1003条带板
商品编号:
Glycaben:1003StripPlate
市场价:
¥0.00
美元价:
0.00
产品分类:
免疫组化试剂盒
公司分类:
Immunostain_SP_Kit
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
GlycatedAlbumin(Glycoalbumin) | |
Manufacturer/Trade: | Exocell/Glycaben |
CatalogueNumbers: | Glycaben:1003StripPlate |
Methodology: | SandwichELISA |
Summaryofprocedure: | Glycabenisanenzymelinkedimmunosorbentassayforthequantitativedeterminationofglycoalbumininhumanplasma.Itprovidesinformationconcerningglycemiccontrolanalogoustothatofferedbyglycohemoglobinmeasurements,exceptthattheproteinmeasuredisglycatedalbuminwhichhasashorterhalf-life. Theassaydependsuponamonoclonalantibody,A717,thatspecificallyrecognizestheglycatedmoietiesinglycatedalbumin.Inpractice,standardsorsamplesareaddedtoA717coatedplates(Glycaben).Theglycoalbuminintheseanalytesbindstooriscapturedbytheantibodyonthesolidphase,andunboundmaterialsarewashedaway.Asubsequentincubationwithanenzymeconjugatedantihumanalbuminantibodyleadstodetectionoftheboundmolecules.BoundconjugateisdeterminedcolorimetricallyafterreactionwithaTMBsubstratesolution. Byrelatingsampleresponsewiththatforthestandards,theconcentrationofglycoalbuminmaybedetermined.Aseparateanalysisoftotalalbuminallowstheresultstobeconvertedtopercentoftotalalbuminthatisglycated. |
SpecimenRequired: | 50ulcitrateorEDTAtreatedplasma |
AssayRange: | 0.1-3mg/mlglycatedalbumin |
Sensitivity: | 0.1mg/ml |
Precision: | Intra-andinterassayprecisionofsampleswithintheassayrangehaveaC.V.<10%. |
Specificity: | ThemonoclonalantibodyA717isspecificforglycatedalbumin,anddoesnotcross-reactwithotherproteinsthatoccurinhumanplasmaincludingnon-glycatedalbumin.Theenzymeconjugatedantibodypreparationisspecificforhumanalbumin. |
ExpectedValues: | Plasmafromnormalnon-diabeticindividualhave0.4-2.4%glycatedalbuminwhereasthatfromuncontrolleddiabeticswillbe>2.5%. |
©2012ExocellInc. |