Exocell/DTNB-Thiols/1021
商品编号:
1021
市场价:
¥7600.00
美元价:
4560.00
产品分类:
免疫组化试剂盒
公司分类:
Immunostain_SP_Kit
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
DTNB-Thiols | |
Manufacturer/Trade: | Exocell/DTNB-Thiols |
CatalogueNumbers: | 1021 |
Methodology: | ChemicalAssay |
Summaryofprocedure: | Thethiolsassaykitmeasuressulfhydrylgroupswiththethiolreagent5-5"-dithiobis[2-nitrobenzoicacid](DTNB),whichformsthe5-thionitrobenzoicacidandamixeddisulfide.Underconditionsofoxidativestress,freesulfhydryldecreasesanddisulfidesincrease.DeterminationofthefreethiolconcentrationinBIOLOGicalsamplesreflectstheABIlitytodetoxifylipidandotherperoxides.Exocell"sDTNB-Thiolsassaycanbeusedwithaspectrumofbiologicalsamplesincludingbodyfluids,tissueandcellspecimens. Tocompletetheassay,aliquotsofstandardorsamplearereactedwithrehydratedDTNBreagentinmicrotiterwells.Absorbanceisreadat405nmusingareferencefilterof~630nm.Concentrationinsamplesisdeterminedfromthestandardcurve. |
SpecimenRequired: | 50uL |
AssayRange: | 0-56.9nmole/well |
Precision: | Intra-andinter-assayprecision<5% |
©2012ExocellInc. |